Lab 02

Components of Plant Cells

Living Contents (Protoplasmic)

Plastids

Chloroplastids (Chloroplasts)

Skill 13

Discoid Chloroplasts

Mount a fresh Elodea leaf in water and examine microscopically to note many discoid Chloroplasts within each cell.

Skill 14

Spiral Chloroplasts

Mount few filaments of Spirogyra in water  and  examine to note spiral chloroplasts, each with many pyrenoids.

Skill 15

Stellate Chloroplasts

Mount few filaments of Zygnema and examine to note two Stellate plastids in every cell; each  one with only one pyrenoid.

Chromoplastids (Chromoplasts)

Skill 16

Globular Bodies

Examine the provided T.S. of pericarp of red fruit Capsicum spp "Pepper" to note that the fruit color is due to definite colored bodies (dense globular & irregular chromoplasts) within cells.

Non-Living Contents

(Non-protoplasmic)

Starch Grains

Skill 17

In Potato Tubers.

Simple

Eccentric Hilum

Skill 18

In Phaseolus seeds.

Simple

Concentric, Branched elongated Hilum

Stratified

Skill 19

In Pisum seeds

Simple

Concentric, Branched Stellate Hilum

Stratified

Skill 20

In Zea grains

Simple

Concentric, branched Hilum

Non-Stratified 

Crystals

Calcium Oxalate

Skill 21

Solitary Crystals "Rombiod-Shaped"

Strip off the epidermis from the fleshy leaf bases of Garlic bulb, spread over the slide, mount in glycerin cover with a cover slip.Examine microscopically the S.V. to note the solitary crystals .

Skill 22

Druses Crystals "Rosette-shaped"

Examine T.S. of Abutilon petiole to note them mainly in the cortical cells.

Skill 23

Raphides Crystals "Needel-like"

Examine T.S in Dracaena stem.

Calcium carbonate

Skill 24

Cystolith

Examine V.S. in Ficus elastica leaf to note these calcium carbonate crystals precipitated on long stalks arising from the outer wall of some epidermal cells "large lithocyst" forming Cystolith.